Cell motility: ARNOand ARF6 at the cutting edge
نویسندگان
چکیده
The transition of epithelial cells from a stationary to a motile state is important in embryogenesis, wound repair and metastasis. Recent studies have shown that ARNO and ARF6 play significant roles in coordinating this transition, providing new insight into the interplay between the Rho and ARF families of GTPases.
منابع مشابه
Betacap73-ARF6 interactions modulate cell shape and motility after injury in vitro.
To understand the role that ARF6 plays in regulating isoactin dynamics and cell motility, we transfected endothelial cells (EC) with HA-tagged ARF6: the wild-type form (WT), a constitutively-active form unable to hydrolyze GTP (Q67L), and two dominant-negative forms, which are either unable to release GDP (T27N) or fail to bind nucleotide (N122I). Motility was assessed by digital imaging micros...
متن کاملThe DOCK180/Elmo Complex Couples ARNO-Mediated Arf6 Activation to the Downstream Activation of Rac1
Cell motility requires extensions of the plasma membrane driven by reorganization of the actin cytoskeleton. Small GTPases, particularly the Rho family, are key regulators of this process. A second class of GTPases, the ADP-ribosylation factors (ARFs), have also been implicated in the regulation of the actin cytoskeleton and motility. ARF6 is intimately involved in the regulation of Rac activit...
متن کاملMetastasis: Wherefore Arf Thou?
The small GTP-binding protein Arf6 is known to be an important regulator of the actin cytoskeleton and of cell motility associated with metastasis. A recent study identifies yet another role for Arf6 in metastasis - as a regulator of plasma-membrane-derived microvesicle release.
متن کاملActivation of ARF6 by ARNO stimulates epithelial cell migration through downstream activation of both Rac1 and phospholipase D
Migration of epithelial cells is essential for tissue morphogenesis, wound healing, and metastasis of epithelial tumors. Here we show that ARNO, a guanine nucleotide exchange factor for ADP-ribosylation factor (ARF) GTPases, induces Madin-Darby canine kidney epithelial cells to develop broad lamellipodia, to separate from neighboring cells, and to exhibit a dramatic increase in migratory behavi...
متن کاملADP Ribosylation Factor 6 Regulates Neuronal Migration in the Developing Cerebral Cortex through FIP3/Arfophilin-1-dependent Endosomal Trafficking of N-cadherin
During neural development, endosomal trafficking controls cell shape and motility through the polarized transport of membrane proteins related to cell-cell and cell-extracellular matrix interactions. ADP ribosylation factor 6 (Arf6) is a critical small GTPase that regulates membrane trafficking between the plasma membrane and endosomes. We herein demonstrated that the knockdown of endogenous Ar...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
- Current Biology
دوره 11 شماره
صفحات -
تاریخ انتشار 2001